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MOLECULAR BIOLOGY: WORKING WITH PROTEINS

PROTEIN-DNA INTERACTIONS

1. Crosslinking DNA-Binding Protein to DNA Using a Bromodeoxyuridine-Substituted Probe
2. Determination of the Molecular Weight of a DNA Binding Protein
3. DNA Band Shift Assay
4. DNA-Dependent Protein Kinase Activity Assay
5. DNase I Footprinting
6. DNase I-Primer Extension Footprinting of Chromatin Assembled on Template DNA
7. Gel Mobility Shift Assay Using Magnesium Chloride and EDTA
8. Nuclear Extract from HGCO/MT Δ2-3 Cells: Parker Extract
9. Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
10. Preparation of End-Labeled DNA Probes by Polymerase Chain Reaction (PCR) for DNA Footprinting Analysis
11. Uracil Interference Assay






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